or placebo o relative to sham instrumented control hearts at top left panel, top right and min bottom left panel mean lvdp of hearts from to min of reperfusion are shown in the bottom right panel tetrazolium extreme caffeine sensitivity chloride stained heart slices showed that hearts treated with csil at , and min had similar injury as that of the sham operated hearts � , � and � , and � respectively p � ns the infarct size of hearts extreme caffeine sensitivity treated with csil even at min of global ischemia was � of the extreme caffeine sensitivity ventricles this was significantly smaller than its corresponding control p , whereas hearts treated with control iggl at , and min of global ischemia were � , � extreme caffeine sensitivity and � respectively fig , left panel the corresponding nitroblue tetrazolium chloride stained heart extreme caffeine sensitivity slices are shown in fig , right panel another parameter of myocardial injury that was determined was mitochondrial size although mitochondrial swelling is a hallmark of ischemic injury, irreversible injury cannot be directly extrapolated from just observation of mitochondrial extreme caffeine sensitivity size nevertheless, in view of the myocardial functional and his tochemical evidences, mitochondrial extreme caffeine sensitivity size assessment from transmission electron micrographs add additional support for myocardial preservation extreme caffeine sensitivity in csil treated hearts, relative to iggl or placebo treated hearts figure shows extreme caffeine sensitivity the comparison of fig mean infarct sizes of rat hearts treated with csil extreme caffeine sensitivity or iggl or placebo at and min of global ischemia left panel the corresponding nitroblue tetrazolium chloride stained mid slices of rat hearts treated with extreme caffeine sensitivity csil or iggl at , and min of global ischemia minimal injury was seen extreme caffeine sensitivity in and min csil treated hearts, but injury was evident in the min extreme caffeine sensitivity csil treated heart slice injury is evident in all heart slices treated extreme caffeine sensitivity with iggl right panel, bottom two rows �i i normal cut csil csil extreme caffeine sensitivity csejo igsl tg& iflsi placebo v fig mean mitochondrial size of normal, csil, iggl or placebo treated hearts treatment was as indicated in the text mitochondrial extreme caffeine sensitivity size of normal hearts, csil treatment at and min of global ischemia, as well as iggl treated hearts at , and min of global ischemia and with placebo no difference in mitochondrial size was observed between normal myocardium � extreme caffeine sensitivity mean number of pixels � sem and myocardium treated at and min of global ischemia � , � , � and � respectively p = ns however, extreme caffeine sensitivity mitochondria of hearts treated with iggl at , and min of global ischemia or extreme caffeine sensitivity placebo � , � , � and � respectively were larger than mitochondria of csil extreme caffeine sensitivity treated hearts p these studies showed that myocardial viability preservation is not extreme caffeine sensitivity restricted to embryonic cardiocytes in cultures adult hearts are also amenable to structural and functional preservation, following cell membrane lesion sealing in a timedependent manner during extreme caffeine sensitivity ischemia this method of cell membrane lesion sealing has also been reported to preserve the integrity of vascular endothelium with antiactinimmunoliposomes a question that remains extreme caffeine sensitivity concerning the utility of csil is whether immunoliposomes can retain the protective functions extreme caffeine sensitivity in the presence of plasma proteins in vivo, since experiments have demonstrated that extreme caffeine sensitivity cells in culture and adult hearts perfused with nonprotein oxygenated buffer were extreme caffeine sensitivity prevented from undergoing myocardial necrosis, following cell membrane lesion sealing intervention with cytoskeletal antigen specific immunoliposomes to demonstrate that cell membrane lesion sealing also occur in extreme caffeine sensitivity vivo, rabbits with experimental myocardial infarction were used in this study, rabbits extreme caffeine sensitivity were injected with antimyosin csil, plain liposomes or saline at the time of left circumflex coronary artery occlusion by intracoronary infusion the occlusion was kept for extreme caffeine sensitivity min followed by hrs of reperfusion the hearts were excised, sliced into � extreme caffeine sensitivity slices parallel to the short axis and stained with nitroblue tetrazolium chloride extreme caffeine sensitivity the infarct was approximately to of the infarcts of the control plain liposome extreme caffeine sensitivity or saline treated rabbit hearts subsequently, comparison to iggliposome treated hearts with acute myocardial infarction demonstrated that the csil treatment resulted in significantly smaller infarct size, extreme caffeine sensitivity as was observed in comparison to plain liposome or saline treated hearts thus, cytoskeletalantigen specific immunoliposomes, consisting of antimyosin or antiactinimmunoliposomes, were demonstrated to be extreme caffeine sensitivity able to preserve cell viability and integrity its potential utility in the cardiovascular extreme caffeine sensitivity system would be enhanced once its efficacy following intravenous delivery has been extreme caffeine sensitivity demonstrated however, the study of asahi et al showed that intravenous delivery of the antiactin immunoliposomes enabled preservation of the integrity of the endothelial cells of extreme caffeine sensitivity the cerebral vessels csil as targeted gene or drug delivery due to the extreme caffeine sensitivity proposed mechanism of cell membrane lesion sealing, we also proposed that if drugs or gene constructs were to be included in the immunoliposomes such as csils, then these drugs or gene constructs should be delivered directly into the cytoplasm fig this route should bypass the endocytic route of drugs or extreme caffeine sensitivity gene construct delivery, thereby reducing destruction of remeron side effect the delivered cargo by the lysosomal extreme caffeine sensitivity enzymes, after formation of endolysosomes using silver grains as model fig transmission electron micrographs of embryonic cardiocyte treated with silver grains impregnated csil left and plain liposome impregnated with silver grains right � = jim drugs, we demonstrated extreme caffeine sensitivity that these drugs can be delivered directly into the cytoplasm of hypoxic cardiocytes treated with silver grains loaded csils figure left shows a transmission electron micrograph extreme caffeine sensitivity of a cardiocyte treated with silver grains impregnated csils silver grains in extreme caffeine sensitivity groups of concentration at about nm were observed however, in cells treated with extreme caffeine sensitivity silver grains impregnated plain liposomes, very few cells were viable of one such cell detected by transmission electron microscopy, the silver grains were observed in the extracellular space [fig right] fig diagrammatic representation of delivery of intraliposomally entrapped extreme caffeine sensitivity genetic construct or drugs directly into the cytoplasm of target cell nmuin ftivoih wiiit mi ��� when the silver grains were replaced with genetic constructs, pgl and psv gal vectors, hypoxic cardiocytes treated with csil impregnated with either vectors extreme caffeine sensitivity showed luciferase activity or bacterial jgalactosidase activity the successful transfection of the extreme caffeine sensitivity hypoxic cardiocytes with pgl, a vector for firefly luciferase enzyme � � fig extreme caffeine sensitivity relative light units of luciferase activity of cardiocytes treated with various preparations and extreme caffeine sensitivity controls in csils is shown in fig as relative light units rlus extreme caffeine sensitivity rlus were determined by the use of a luminometer as can be seen, only hypoxic cardiocytes treated with pglcsils showed increased rlus significantly above normal cells extreme caffeine sensitivity with treatment with no vectors similarly, normoxic cardiocytes treated with pglcsil, hypoxic cardiocytes extreme caffeine sensitivity and normoxic cardiocytes treated with plain liposomes, or with only vectors, showed extreme caffeine sensitivity no significant gene transcription and expression when hypoxic cardiocytes were treated with csil with entrapped psvgal vectors, almost all cells in the field of view under extreme caffeine sensitivity light microscopy exhibited bacterial �galactosidase enzyme activity, following reaction with xgal bromo extreme caffeine sensitivity chloroindolylbetadgalactopyranoside, nm mgcl,mm iqfetcn h, mm kfecn in phosphate buffered saline ph [fig a] when this mode of gene expression was compared with transfection of extreme caffeine sensitivity psvjgal vector with cationic liposomes, cationic liposome transfection according to the manufacturers protocol resulted in transfection of only a few cells per field of view extreme caffeine sensitivity [fig b], in this micrograph, two cells with intense ��galactosidase activity were observed quantitation of the number of cells in the field of view that was extreme caffeine sensitivity successfully trans fected with psvjgal vector in csil, cationic liposome, iggliposomes, plain liposome and vector alone are shown in fig c only csil and cationic transfection showed gene expression csiltransfection or csilfection was more than times more efficient extreme caffeine sensitivity in transfecting cells than cationic liposomes although the intensity of gene expression was extreme caffeine sensitivity low with csilfection, using the initial vector concentration of xg of vectors extreme caffeine sensitivity in mg lipids in ml, when the vector concentration was increased to � zg, also in mg lipids in ml, the intensity of gene transfection was extreme caffeine sensitivity increased [fig d], this study showed that approximately x xg ��� � m t� !